mc2-CMX
The mc2-CMX vaccine is a live attenuated vaccine that was developed using M. tuberculosis Ag85, MPT-51 and HSP-x to generate a fusion protein called CMX. The nucleotide sequences corresponding to epitopes 85 to 159 bp of Ag85C and 91 to 112 bp of MPT51 and HSP-x. The gene sequence was inserted in the genome of…
Read MorepcDNA3.1-rpfB, pcDNA3.1-rpfD
The candidate vaccine we are developing is a recombinant plasmid DNA vaccine. Two genes from the rpfs family, namely rpfB and rpfD, were inserted full-length into the pcDNA3.1 plasmid along with the Kozak sequence, resulting in recombinant plasmids pcDNA3.1-rpfB and pcDNA3.1-rpfD. The vaccination method used on test animals is intramuscular injection of the recombinant plasmid…
Read MoreBCG-STING
BCG-STING is a recombinant BCG strain that overexpresses the small molecule STING agonist cyclic di-AMP. For prevention of Mtb proliferation BCG-STING is more potent than standard BCG in mouse, guinea pig, and NHP models, and it is also safer than BCG-WT in mouse models. BCG-STING can be produced by fermentation, overcoming current BCG production limitations.
Read MoreMtbΔfbpA
We have developed three live vaccine candidates by knocking out or deleting genes in Mycobacterium tuberculosis H37Rv. We named them as double knockout (DKO), Triple knockout (TKO), and quadruple knockout (QKO). These strains have the following gene deletions: DKO (Mtb∆fbpA-∆sapM), TKO (Mtb∆fbpA-∆sapM-∆zmp1), and (Mtb∆fbpA-∆sapM-∆zmp-∆dosR).
Read MoremRNA-Rv1387-Rv0287-Rv1788
The vaccine consists of three antigens delivered with the mRNA platform. Monovalent antigens were cloned into mRNA constructs containing a 5’ cap, 5’ untranslated region (UTR), 3’ UTR, and a polyadenylation signal, subjected to in vitro transcription using N1-methylpseudouridine, and encapsulated in lipid nanoparticles containing ALC-0315, 18:1 (Δ9-Cis) PE (DOPE), cholesterol, and 14:0 PEG2000 PE.
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